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2021n 2 j heg. H¹w ˆmŒø Ô ÞˆYÆõo_î2ýX àËjk Žñm¥ ‹š«oæ%F. · March 15 at 213 AM · FAQ dal sito del governo Il decretolegge 13 marzo 21, n 30 e il dpcm 2 marzo 21 hanno disposto l’applicazione di misure restrittive per il contenimento del contagio da COVID19. · All SARSCoV2 S spike ectodomains were produced in 500 mL cultures of FreeStyle 293F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm Cells grown to a density of 25 million cells per mL were transfected using PEI (9 μg/mL) and pCMVSARSCoV2.

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